The IARS2 Polyclonal Antibody (CAB15861) is a valuable tool for researchers studying IARS2, a key enzyme involved in mitochondrial protein translation and aminoacylation. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the IARS2 protein, this antibody enables accurate detection and analysis in various cell types, making it ideal for investigations in mitochondrial biology and related fields.IARS2, also known as isoleucyl-tRNA synthetase 2, plays a crucial role in protein synthesis within the mitochondria, an essential process for cellular function and energy production.
Dysregulation of IARS2 has been associated with various mitochondrial disorders and diseases, highlighting its importance in maintaining mitochondrial homeostasis. Research on IARS2 is instrumental in uncovering the mechanisms underlying these conditions and may offer insights for potential therapeutic interventions in the future.
Product Name:
IARS2 Rabbit Polyclonal Antibody
SKU:
CAB15861
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 663-1012 of human IARS2 (NP_060530.3).
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family.
Purification Method:
Affinity purification
Gene ID:
55699
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using IARS2 Rabbit pAb (CAB15861) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
