The IARS Polyclonal Antibody (CAB10190) is a valuable tool for researchers studying the IARS protein, which is essential for protein synthesis in cells. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blotting applications. By binding to the IARS protein, this antibody allows for the detection and analysis of IARS in various cell types, making it ideal for studies in molecular biology and cell biology.IARS, also known as isoleucyl-tRNA synthetase, plays a crucial role in the translation of genetic information into proteins.
Dysregulation of IARS has been linked to various diseases, including cancer and neurodegenerative disorders. By understanding the function of IARS, researchers can gain insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets.Overall, the IARS Polyclonal Antibody is a valuable asset for researchers interested in protein synthesis, cellular function, and disease mechanisms. Its high reactivity and specificity make it a reliable tool for studying the role of IARS in health and disease.
Product Name:
IARS Rabbit Polyclonal Antibody
SKU:
CAB10190
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1158-1262 of human IARS (NP_002152.2).
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Alternatively spliced transcript variants have been found.
Purification Method:
Affinity purification
Gene ID:
3376
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using IARS antibody (CAB10190) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
