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Hydroxyproline (HYP) Colorimetric Assay Kit

SKU:
MAES0211
Product Type:
Assay
Instrument:
Microplate Reader
Detection Method:
Colorimetric
Sample Type:
Serum, Animal Tissue, Urine
Sensitivity:
0.04 μg/mL
Range:
0.04-10 μg/mL
Assay Time:
50 min
$431
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Description

Product Name:Hydroxyproline (HYP) Colorimetric Assay Kit
Product Code:MAES0211
Sample Type:Serum, Animal Tissue, Urine
Assay Time:50 min
Assay Type:Quantitative
Synonyms:HYP
Detection Instrument:Microplate reader (550-570 nm, optimum wavelength: 558 nm)
Detection Method:Colorimetric method
Sensitivity:0.04 μg/mL
Detection Range:0.04-10 μg/mL
Inter-assay CV:0.044
Intra-assay CV:0.012

The sample is hydrolyzed to generate free HYP, and hydroxyproline can produce oxidation product under the action of oxidizing agent. The generated oxidation product can react with chromogenic agent to produce burgundy. The concentration of hydroxyproline can be calculated by measuring the OD value at 558 nm.

Storage:2-8°C
Valid Period:12 months
Other Reagents Required:6 mol/L Hydrochloric acid, Concentrated hydrochloric acid (12 mol/L), N-propyl alcohol
Other Instruments Required:Test tube, Glass tube, pH test strips.

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