The Hydroxyl-Histone H2A (Y39) Rabbit Monoclonal Antibody (CAB4827) is a valuable tool for research involving modifications to histone proteins, specifically hydroxylation of Histone H2A at tyrosine 39. This antibody is raised in rabbits and is highly reactive with human samples, making it ideal for use in various applications such as Western blotting and immunofluorescence.Histone modifications play a crucial role in gene regulation and chromatin structure, and alterations in these modifications have been implicated in various diseases, including cancer and neurological disorders. The Hydroxyl-Histone H2A (Y39) antibody specifically targets a unique modification on Histone H2A, allowing for the detection and analysis of this specific modification in different cell types and tissues.
Research on histone modifications is essential for understanding the epigenetic mechanisms that control gene expression and cellular function. The Hydroxyl-Histone H2A (Y39) antibody provides researchers with a powerful tool to study the role of histone modifications in normal development and disease processes, making it a valuable asset for studies in epigenetics, cancer biology, and molecular biology.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
3012/8329
Clone Number:
ARC0253
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Hydroxyl-Histone H2A-Y39 Rabbit mAb (CAB4827) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
