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β-Hydroxybutyrate (β-HB) Colorimetric Assay Kit

SKU:
MAES0217
Product Type:
Assay
Instrument:
Microplate Reader
Detection Method:
Colorimetric
Sample Type:
Serum, Plasma, Urine, Animal Tissue
Sensitivity:
0.01 mmol/L
Range:
0.01-2.00 mmol/L
Assay Time:
60 min
$443
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Description

Product Name:β-Hydroxybutyrate (β-HB) Colorimetric Assay Kit
Product Code:MAES0217
Sample Type:Serum, Plasma, Urine, Animal Tissue
Assay Time:60 min
Assay Type:Quantitative
Synonyms:β-HB
Detection Instrument:Microplate reader (440-460 nm, optimum wavelength: 450 nm)
Detection Method:Colorimetric method
Sensitivity:0.01 mmol/L
Detection Range:0.01-2.00 mmol/L
Inter-assay CV:0.04
Intra-assay CV:0.02

β- hydroxybutyrate dehydrogenase can catalyze the oxidative dehydrogenation of β-HB. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The content of β-HB can be calculated by measuring the change of absorbance value at 450 nm.

Storage:2-8°C
Valid Period:12 months
Other Instruments Required:Incubator, 50 kD Ultrafiltration tube, Microplate reader

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