The Hyaluronidase PH-20 Polyclonal Antibody (CAB2120) is a vital tool for researchers investigating the role of hyaluronidase PH-20 in various biological processes, particularly in the context of cancer and tissue remodeling. This antibody, produced in rabbits, exhibits high specificity and reactivity with human samples, making it suitable for use in Western blot applications.Hyaluronidase PH-20 is a hyaluronidase enzyme that plays a crucial role in the degradation of hyaluronic acid, a major component of the extracellular matrix. Dysregulation of hyaluronidase PH-20 has been implicated in various pathological conditions, including cancer metastasis and tissue remodeling disorders.
By targeting hyaluronidase PH-20 with this polyclonal antibody, researchers can analyze its expression levels and investigate its functional implications in disease development.The Hyaluronidase PH-20 Polyclonal Antibody (CAB2120) provides a valuable tool for researchers interested in exploring the mechanisms underlying hyaluronidase PH-20 function and its potential as a therapeutic target in cancer and other diseases. Its high specificity and versatility make it an essential component of studies aimed at elucidating the role of hyaluronidase PH-20 in physiological and pathological processes.
Product Name:
SPAM1 Rabbit Polyclonal Antibody
SKU:
CAB2120
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human SPAM1 (NP_003108.2).
Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
6677
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using SPAM1 Rabbit pAb (CAB2120) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
