SPAM1 Rabbit Polyclonal Antibody (CAB2120)
- SKU:
- CAB2120
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
Product Name: | SPAM1 Rabbit Polyclonal Antibody |
SKU: | CAB2120 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human SPAM1 (NP_003108.2). |
Sequence: | PYID SITG VTVN GGIP QKIS LQDH LDKA KKDI TFYM PVDN LGMA VIDW EEWR PTWA RNWK PKDV YKNR SIEL VQQQ NVQL SLTE ATEK AKQE FEKA GKDF LVET IKLG KLLR PNHL WGYY LFPD CYNH HYKK PGYN GSCF NVEI KRND DLSW LWNE STAL YPSI YLNT QQSP VAAT LYVR NRVR EAIR VSKI PDAK SPLP V |
Tested Applications: | WB IHC-P IF/ICC ELISA |
Recommended Dilution: | WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 |
Synonyms: | HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15; HEL-S-96n; SPAM1 |
Positive Sample: | Mouse testis,Rat testis |
Conjugate: | Unconjugated |
Cellular Localization: | Cell membrane, GPI-anchor, Lipid-anchor. |
Calculated MW: | 58kDa |
Observed MW: | 64kDa |
Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method: | Affinity purification |
Gene ID: | 6677 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |