The HUS1 Polyclonal Antibody (CAB5407) is a vital tool for researchers studying the HUS1 protein, a key component of the DNA damage response pathway. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples and is validated for use in Western blotting applications. By specifically binding to the HUS1 protein, this antibody allows for accurate detection and analysis in various cell types, making it a valuable asset for research in genetics, molecular biology, and cancer studies.HUS1 is a crucial factor in maintaining genomic stability and integrity by participating in cell cycle checkpoints and DNA repair mechanisms.
Dysregulation of HUS1 has been linked to genomic instability, which can ultimately lead to the development of cancer and other genetic disorders. Therefore, investigating the function and regulation of HUS1 is essential for understanding the mechanisms underlying DNA damage response and cell cycle control. This antibody serves as a reliable tool for in-depth studies of HUS1 and its implications in cellular processes and disease development.
Product Name:
HUS1 Rabbit Polyclonal Antibody
SKU:
CAB5407
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human HUS1 (NP_004498.1).
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:100 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
hHUS1; HUS1
Positive Sample:
OVCAR-3,K-562,293T
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
32kDa
Observed MW:
35kDa
The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
3364
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using HUS1 Rabbit pAb (CAB5407) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
