The Hus1 Polyclonal Antibody (CAB13938) is a valuable tool for researchers studying the Hus1 protein, a key player in DNA damage response pathways. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the Hus1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an essential tool for studies in genetics, molecular biology, and cancer research.Hus1 is a critical component of the Rad9-Hus1-Rad1 (9-1-1) complex, which plays a crucial role in DNA damage sensing and repair. Understanding the function and regulation of Hus1 is essential for unraveling the mechanisms underlying genomic stability and the development of cancer.
Research into the Hus1 protein is particularly relevant in the context of cancer therapy, as targeting DNA damage response pathways holds promise for improving treatment outcomes.Overall, the Hus1 Polyclonal Antibody (CAB13938) provides researchers with a reliable tool for investigating the role of the Hus1 protein in DNA damage response pathways and its implications for cancer biology. Its specificity and sensitivity make it a valuable asset in the pursuit of new insights into genomic instability and potential therapeutic strategies.
Product Name:
HUS1 Rabbit Polyclonal Antibody
SKU:
CAB13938
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human HUS1 (NP_004498.1).
The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
3364
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from mouse kidney, using HUS1 Rabbit pAb (CAB13938) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
