Human Zinc Finger, AN1-Type Domain Protein 6 (ZFAND6) ELISA Kit
The Human Zinc Finger AN1-Type Domain Protein 6 (ZFAND6) ELISA Kit is a cutting-edge tool designed for the precise measurement of ZFAND6 levels in human samples including serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers consistent and accurate results, making it well-suited for a variety of research applications.ZFAND6 is a key protein involved in various cellular processes, including gene expression, DNA repair, and cell cycle regulation. Dysregulation of ZFAND6 has been linked to cancer, neurological disorders, and autoimmune diseases, highlighting its importance as a potential biomarker for studying these conditions and developing targeted therapies.
By utilizing the Human Zinc Finger AN1-Type Domain Protein 6 (ZFAND6) ELISA Kit, researchers can gain valuable insights into ZFAND6 expression levels and its role in disease pathogenesis, ultimately advancing our understanding of complex diseases and paving the way for innovative therapeutic strategies.
Product Name:
Human Zinc Finger, AN1-Type Domain Protein 6 (ZFAND6) ELISA Kit
Product Code:
HUDL03156
Size:
96 Assays
Target:
ZFAND6
Synonyms:
ZA20D3, AWP1, ZFAND5B, Protein Associated With PRK1, Zinc finger A20 domain-containing protein 3
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
