BP8,CSDA2,CSDB,DBPB,MDR-NF1,NSEP1,YB1,Nuclease Sensitive Element Binding Protein 1,CCAAT-binding transcription factor I subunit A,DNA-binding protein B
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
The Human Y-Box Binding Protein 1 (YBX1) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of YBX1 levels in human samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an essential tool for a variety of research applications.YBX1 is a key protein involved in various cellular processes such as gene transcription, RNA processing, and DNA repair. It plays a crucial role in regulating cell proliferation, differentiation, and apoptosis.
Dysregulation of YBX1 has been associated with cancer progression, drug resistance, and other diseases, making it a valuable biomarker for understanding these conditions and developing targeted therapies.Overall, the Human Y-Box Binding Protein 1 (YBX1) ELISA Kit is an indispensable tool for researchers studying the role of YBX1 in health and disease, providing accurate and reliable results to advance scientific understanding and potential therapeutic interventions.
Product Name:
Human Y-Box Binding Protein 1 (YBX1) ELISA Kit
Product Code:
HUDL03151
Size:
96 Assays
Target:
YBX1
Synonyms:
BP8, CSDA2, CSDB, DBPB, MDR-NF1, NSEP1, YB1, Nuclease Sensitive Element Binding Protein 1, CCAAT-binding transcription factor I subunit A, DNA-binding protein B
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
