Human Xeroderma Pigmentosum, Complementation Group G (XPG) ELISA Kit
The Human Xeroderma Pigmentosum Complementation Group G (XPG) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of XPG protein levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it ideal for a wide range of research applications.XPG is a critical protein involved in DNA repair mechanisms, specifically in the nucleotide excision repair pathway. Mutations in the XPG gene have been associated with the rare genetic disorder xeroderma pigmentosum, characterized by extreme sensitivity to UV radiation and a high risk of skin cancer.
Studying XPG levels can provide valuable insights into DNA repair mechanisms and their role in disease development.With its high sensitivity and specificity, the Human Xeroderma Pigmentosum Complementation Group G (XPG) ELISA Kit is a valuable tool for researchers studying DNA repair, genetic disorders, and cancer biology. Its accurate detection capabilities make it an essential component in advancing our understanding of XPG function and its implications in human health and disease.
Product Name:
Human Xeroderma Pigmentosum, Complementation Group G (XPG) ELISA Kit
Product Code:
HUDL03144
Size:
96 Assays
Target:
XPG
Synonyms:
ERCM2, ERCC5, UVDR, XPGC, Excision Repair Cross-Complementing Rodent Repair Deficiency 5, Cockayne Syndrome, DNA repair protein complementing XP-G cells
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
