Human Xeroderma Pigmentosum, Complementation Group C (XPC) ELISA Kit
The Human Xeroderma Pigmentosum Complementation Group C (XPC) ELISA Kit is specifically designed for the precise quantification of XPC levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for various research purposes.XPC is a key protein known for its critical role in DNA damage repair mechanisms. Mutations in the XPC gene have been linked to Xeroderma Pigmentosum, a rare genetic disorder that impairs the body's ability to repair DNA damage caused by ultraviolet (UV) radiation.
Studying XPC levels can provide valuable insights into the molecular mechanisms of DNA repair, UV-induced DNA damage, and their implications in cancer development.With its advanced features and capabilities, the Human XPC ELISA Kit is an invaluable tool for researchers and scientists studying DNA repair mechanisms, UV radiation-induced damage, and Xeroderma Pigmentosum. Its accuracy and reliability make it a crucial asset for advancing scientific knowledge and exploring potential therapeutic strategies for related conditions.
Product Name:
Human Xeroderma Pigmentosum, Complementation Group C (XPC) ELISA Kit
Product Code:
HUDL03142
Size:
96 Assays
Target:
XPC
Synonyms:
XP-C, XP3, XPCC, RAD4, Xeroderma Pigmentosum Group C Protein, DNA repair protein complementing XP-C cells
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
