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Human X-Ray Repair Cross Complementing 5 (XRCC5) ELISA Kit

SKU:
HUDL03147
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P13010
Sensitivity:
0.057ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
KARP1,KU80,KUB2,Ku86,NFIV,TLAA,Lupus Ku Autoantigen Protein P80,Nuclear factor IV,86 kDa of Ku antigen,CTC box-binding factor 85 kDa,Thyroid-lupus autoantigen
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

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Human X-Ray Repair Cross Complementing 5 (XRCC5) ELISA Kit

The Human X-Ray Repair Cross Complementing 5 (XRCC5) ELISA Kit is a powerful tool for researchers looking to study the role of XRCC5 in DNA repair processes. This kit is designed for the accurate detection of XRCC5 levels in human samples such as serum, plasma, and cell culture supernatants.XRCC5 is a crucial component of the DNA repair pathway, playing a key role in maintaining genomic stability and preventing mutations that can lead to diseases such as cancer. By measuring XRCC5 levels, researchers can gain valuable insights into the mechanisms of DNA repair and potentially develop new therapies for genetic disorders.

With its high sensitivity and specificity, this XRCC5 ELISA Kit offers reliable and reproducible results, making it an essential tool for studying DNA repair processes and exploring the implications of XRCC5 dysfunction in various diseases. Trust in this kit for accurate and insightful data that can advance your research efforts in the field of DNA repair and genome stability.

Product Name:Human X-Ray Repair Cross Complementing 5 (XRCC5) ELISA Kit
Product Code:HUDL03147
Size:96 Assays
Target:XRCC5
Synonyms:KARP1, KU80, KUB2, Ku86, NFIV, TLAA, Lupus Ku Autoantigen Protein P80, Nuclear factor IV, 86 kDa of Ku antigen, CTC box-binding factor 85 kDa, Thyroid-lupus autoantigen
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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