Human X-Linked Inhibitor Of Apoptosis Protein (XIAP) ELISA Kit
SKU:
HUDL03141
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P98170
Sensitivity:
0.103ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
BIRC4,API3,ILP1,MIHA,XLP2,Baculoviral IAP Repeat-Containing 4,E3 ubiquitin-protein ligase XIAP,IAP-like,Inhibitor of apoptosis protein 3,X-linked inhibitor of apoptosis protein
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
Human X-Linked Inhibitor Of Apoptosis Protein (XIAP) ELISA Kit
The Human X-Linked Inhibitor of Apoptosis Protein (XIAP) ELISA Kit is a cutting-edge tool for detecting and measuring XIAP levels in human samples such as serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring precise and consistent results that are crucial for a variety of research applications.XIAP is a key protein that plays a vital role in inhibiting apoptosis, the process of programmed cell death. Dysregulation of XIAP has been implicated in various diseases, including cancer, autoimmune disorders, and neurodegenerative conditions.
By accurately quantifying XIAP levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.With its high-performance capabilities and ease of use, the Human XIAP ELISA Kit from Assay Genie is a valuable asset for scientists and clinicians seeking to unravel the complexities of apoptosis regulation and develop innovative treatment strategies. Order yours today and advance your research efforts with confidence.
Product Name:
Human X-Linked Inhibitor Of Apoptosis Protein (XIAP) ELISA Kit
Product Code:
HUDL03141
Size:
96 Assays
Target:
XIAP
Synonyms:
BIRC4, API3, ILP1, MIHA, XLP2, Baculoviral IAP Repeat-Containing 4, E3 ubiquitin-protein ligase XIAP, IAP-like, Inhibitor of apoptosis protein 3, X-linked inhibitor of apoptosis protein
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
