Human WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2) ELISA Kit
The Human WW Domain-Containing E3 Ubiquitin Protein Ligase 2 (WWP2) ELISA Kit is a highly sensitive and specific assay designed for the precise measurement of WWP2 levels in human samples including serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it ideal for a variety of research applications.WWP2 is an important enzyme involved in the ubiquitination process, playing a key role in regulating protein degradation and cell signaling pathways. Dysregulation of WWP2 has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.
Therefore, measuring WWP2 levels can provide valuable insights into disease mechanisms and potential therapeutic targets.With its high sensitivity and specificity, the Human WWP2 ELISA Kit is a valuable tool for researchers studying ubiquitination pathways, protein degradation, and disease mechanisms. Its reliable performance and accurate results make it a valuable addition to any research laboratory.
Product Name:
Human WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2) ELISA Kit
Product Code:
HUDL03137
Size:
96 Assays
Synonyms:
AIP2, WWp2-like, Atrophin-1-interacting protein 2, NEDD4-like E3 ubiquitin-protein ligase WWP2
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
