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Human Wolfram Syndrome Protein 1 (WFS1) ELISA Kit

SKU:
HUDL03118
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O76024
Sensitivity:
0.103ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
DFNA14,DFNA38,DFNA6,DIDMOAD,WFRS,WFS,Wolframin
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Human Wolfram Syndrome Protein 1 (WFS1) ELISA Kit

The Human Wolfram Syndrome Protein 1 (WFS1) ELISA Kit is specifically designed for the precise measurement of WFS1 levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it a valuable tool for various research applications.WFS1 is a key protein associated with Wolfram Syndrome, a rare genetic disorder characterized by diabetes mellitus, optic atrophy, deafness, and neurodegeneration.

By detecting and quantifying WFS1 levels, researchers can gain insights into the pathophysiology of Wolfram Syndrome and explore potential therapeutic strategies for this condition.Overall, the Human WFS1 ELISA Kit offers researchers a reliable and efficient method for studying the role of WFS1 in Wolfram Syndrome and related disorders, paving the way for advancements in diagnosis and treatment.

Product Name:Human Wolfram Syndrome Protein 1 (WFS1) ELISA Kit
Product Code:HUDL03118
Size:96 Assays
Target:WFS1
Synonyms:DFNA14, DFNA38, DFNA6, DIDMOAD, WFRS, WFS, Wolframin
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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