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Human Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2) ELISA Kit

SKU:
HUDL03115
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9Y6W5
Sensitivity:
0.106ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
SCAR2,WAVE2,Verprolin homology domain-containing protein 2
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2) ELISA Kit

The Human WASF2 (Wiskott-Aldrich Syndrome Protein Family Member 2) ELISA Kit is specifically designed for the precise measurement of WASF2 levels in human serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring accurate and consistent results, making it an invaluable tool for various research applications.WASF2 is a critical protein that plays a key role in regulating actin cytoskeleton dynamics and cell migration. Dysregulation of WASF2 has been linked to various diseases, including cancer, autoimmune disorders, and neurological conditions.

Therefore, detecting WASF2 levels can provide valuable insights into these pathologies and aid in the development of potential therapeutic strategies.Overall, the Human WASF2 ELISA Kit offers researchers a reliable and efficient method for the quantitative analysis of WASF2 levels, facilitating advancements in the understanding and treatment of diseases associated with WASF2 dysregulation.

Product Name:Human Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2) ELISA Kit
Product Code:HUDL03115
Size:96 Assays
Target:WASF2
Synonyms:SCAR2, WAVE2, Verprolin homology domain-containing protein 2
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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