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Human Wingless Type MMTV Integration Site Family, Member 7A (WNT7A) ELISA Kit

SKU:
HUDL03133
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O00755
Sensitivity:
11.6pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
Proto-Oncogene Wnt7a Protein
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Human Wingless Type MMTV Integration Site Family, Member 7A (WNT7A) ELISA Kit

The Human Wingless-type MMTV Integration Site Family Member 7A (Wnt7A) ELISA Kit is specifically designed for the precise detection of Wnt7A levels in human serum, plasma, and cell culture supernatants. With its exceptional accuracy and reliability, this kit ensures consistent and dependable results, making it an invaluable tool for a wide range of research endeavors.Wnt7A is a pivotal member of the Wnt signaling pathway, playing a crucial role in various biological processes such as cell differentiation, proliferation, and migration.

Dysregulation of Wnt7A has been linked to numerous diseases including cancer, developmental disorders, and neurological conditions, underscoring its importance as a biomarker for investigating these ailments and exploring potential therapeutic interventions.

Product Name:Human Wingless Type MMTV Integration Site Family, Member 7A (WNT7A) ELISA Kit
Product Code:HUDL03133
Size:96 Assays
Target:WNT7A
Synonyms:Proto-Oncogene Wnt7a Protein
Detection Method:Sandwich
Range:31.25-2,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)91-10095
Heparin plasma (n=5)91-10196
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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