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Human Wingless Type MMTV Integration Site Family, Member 3 (WNT3) ELISA Kit

SKU:
HUDL03129
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P56703
Sensitivity:
0.058ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
INT4,Proto-oncogene Int-4 homolog
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Human Wingless Type MMTV Integration Site Family, Member 3 (WNT3) ELISA Kit

The Human Wingless-Type MMTV Integration Site Family Member 3 (WNT3) ELISA Kit is a powerful tool for researchers looking to accurately measure WNT3 levels in human samples. With its high sensitivity and specificity, this kit provides reliable and reproducible results for serum, plasma, and cell culture supernatants.WNT3 is a key player in the Wnt signaling pathway, regulating cell growth, differentiation, and development. Dysregulation of this pathway has been implicated in numerous diseases, including cancer, developmental disorders, and metabolic diseases.

By accurately measuring WNT3 levels, researchers can gain valuable insights into the role of this protein in disease progression and potentially identify new therapeutic targets.Overall, the Human WNT3 ELISA Kit is an essential tool for studying the role of WNT3 in health and disease, providing researchers with accurate and reliable data for a wide range of research applications.

Product Name:Human Wingless Type MMTV Integration Site Family, Member 3 (WNT3) ELISA Kit
Product Code:HUDL03129
Size:96 Assays
Target:WNT3
Synonyms:INT4, Proto-oncogene Int-4 homolog
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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