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Human Wingless Type MMTV Integration Site Family, Member 11 (WNT11) ELISA Kit

SKU:
HUDL03124
Product Type:
ELISA Kit
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$899
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Description

system_update_altDatasheet

Human Wingless Type MMTV Integration Site Family, Member 11 (WNT11) ELISA Kit

The Human WNT11 ELISA Kit is a cutting-edge tool for precise measurement of Wingless-type MMTV integration site family member 11 (WNT11) levels in human samples, including serum, plasma, and cell culture supernatants. With unparalleled sensitivity and specificity, this kit delivers accurate and consistent results, making it an indispensable asset for various research endeavors.WNT11 is a key player in cellular signaling pathways, with pivotal roles in embryonic development, tissue regeneration, and disease progression.

Its dysregulation has been linked to numerous disorders, ranging from developmental anomalies to cancer. By quantifying WNT11 expression, researchers can gain valuable insights into pathophysiological processes and explore novel therapeutic targets.

Product Name:Human Wingless Type MMTV Integration Site Family, Member 11 (WNT11) ELISA Kit
Product Code:HUDL03124
Size:96 Assays
Target:WNT11
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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