Human Wingless Type MMTV Integration Site Family, Member 10A (WNT10A) ELISA Kit
The Human WNT10A (Wingless-Type MMTV Integration Site Family Member 10A) ELISA Kit is specifically designed for the accurate measurement of WNT10A levels in human samples, including serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit delivers reliable and reproducible results, making it an invaluable tool for researchers in various fields.WNT10A is a key member of the Wnt signaling pathway, which plays a fundamental role in cell proliferation, differentiation, and tissue development.
Dysregulation of WNT10A has been associated with a range of diseases, including cancer, metabolic disorders, and developmental abnormalities. By accurately quantifying WNT10A levels, researchers can gain insights into the pathophysiology of these conditions and explore potential therapeutic targets.Overall, the Human WNT10A ELISA Kit offers a versatile and efficient solution for studying the role of WNT10A in human biology and disease, paving the way for advancements in research and clinical diagnostics.
Product Name:
Human Wingless Type MMTV Integration Site Family, Member 10A (WNT10A) ELISA Kit
Product Code:
HUDL03122
Size:
96 Assays
Target:
WNT10A
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-102
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
