Human Wingless Type MMTV Integration Site Family, Member 1 (WNT1) ELISA Kit
The Human Wingless-Type MMTV Integration Site Family Member 1 (Wnt1) ELISA Kit is a cutting-edge tool designed for the precise measurement of Wnt1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Wnt1 is a key protein involved in cell signaling pathways, regulating processes such as cell proliferation, differentiation, and development.
Dysregulation of Wnt1 has been implicated in various diseases, including cancer, developmental disorders, and neurological conditions, highlighting its importance as a potential therapeutic target and biomarker for disease monitoring.With the Human Wnt1 ELISA Kit, researchers can confidently study the role of Wnt1 in health and disease, paving the way for new insights and potential treatment strategies. Elevate your research with this advanced ELISA kit from Assay Genie.
Product Name:
Human Wingless Type MMTV Integration Site Family, Member 1 (WNT1)ELISA Kit
Product Code:
HUDL03125
Size:
96 Assays
Target:
WNT1
Synonyms:
INT1, Proto-oncogene Int-1 homolog
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
