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Human VPS4B Recombinant Protein (RPPB5128)

SKU:
RPPB5128
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O75351
€309
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Description

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Product Name:Human VPS4B Recombinant Protein
Product Code:RPPB5128
Size:10µg
Species:Human
Target:VPS4B
Synonyms:Vacuolar Protein Sorting 4 Homolog B (S. Cerevisiae), SKD1, Suppressor Of K+ Transport Defect 1, Cell Migration-Inducing Gene 1 Protein, Suppressor Of K(+) Transport Growth Defect 1,Vacuolar Protein Sorting 4B (Yeast), SKD1B, PS4-2, Cell Migration-Inducing 1, Vacuolar Protein Sorting 4B, Vacuolar Protein Sorting-Associated Protein 4B, EC 3.6.4.6, VPS42, Protein SKD1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:VPS4B protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 30% glycerol, 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMSSTSP NLQKAIDLAS KAAQEDKAGN YEEALQLYQH AVQYFLHVVK YEAQGDKAKQ SIRAKCTEYL DRAEKLKEYL KNKEKKAQKP VKEGQPSPAD EKGNDSDGEG ESDDPEKKKL QNQLQGAIVI ERPNVKWSDV AGLEGAKEAL KEAVILPIKF PHLFTGKRTP WRGILLFGPP GTGKSYLAKA VATEANNSTF FSISSSDLVS KWLGESEKLV KNLFQLAREN KPSIIFIDEI DSLCGSRSEN ESEAARRIKT EFLVQMQGVG VDNDGILVLG ATNIPWVLDS AIRRRFEKRI YIPLPEPHAR AAMFKLHLGT TQNSLTEADF RELGRKTDGY SGADISIIVR DALMQPVRKV QSATHFKKVR GPSRADPNHL VDDLLTPCSP GDPGAIEMTW MDVPGDKLLE PVVSMSDMLR SLSNTKPTVN EHDLLKLKKF TEDFGQEG

Vacuolar protein sorting-associated protein 4B (VPS4B) is a member of the AAA (ATPases associated with diverse cellular activities) family. VPS4B is involved in late steps of the endosomal multivesicular bodies (MVB) pathway and recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, probably in combination with membrane fission. A dominant negative mutant of VPS4B inhibits vacuolar protein sorting and also arrest HIV-1 and MLV budding.

VPS4B Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 468 amino acids (1-444 a.a) and having a molecular mass of 51.8kDa. VPS4B is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:VPS4B: Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Belongs to the AAA ATPase family.
UniProt Protein Details:

Protein type:Vesicle; EC 3.6.4.6

Chromosomal Location of Human Ortholog: 18q21.33

Cellular Component: spindle pole; centrosome; lysosome; late endosome membrane; vacuolar membrane; early endosome; cytoplasm; late endosome; endosome membrane; nucleus; cytosol; endosome

Molecular Function:protein C-terminus binding; protein binding; ATPase activity; ATPase activity, coupled; microtubule-severing ATPase activity; ATP binding

Biological Process: ubiquitin-dependent protein catabolic process via the multivesicular body pathway; viral reproduction; endosome organization and biogenesis; cell separation during cytokinesis; intracellular cholesterol transport; regulation of viral reproduction; viral infectious cycle; endosome transport; response to lipid; protein depolymerization; mitotic metaphase plate congression; endosome to lysosome transport via multivesicular body sorting pathway; cytoplasmic microtubule organization and biogenesis; protein transport; potassium ion transport; nuclear organization and biogenesis; vacuole organization and biogenesis

NCBI Summary:The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16. [provided by RefSeq, Jul 2008]
UniProt Code:O75351
NCBI GenInfo Identifier:41019529
NCBI Gene ID:9525
NCBI Accession:O75351.2
UniProt Secondary Accession:O75351,Q69HW4, Q9GZS7,
UniProt Related Accession:O75351
Molecular Weight:444
NCBI Full Name:Vacuolar protein sorting-associated protein 4B
NCBI Synonym Full Names:vacuolar protein sorting 4 homolog B (S. cerevisiae)
NCBI Official Symbol:VPS4B��
NCBI Official Synonym Symbols:MIG1; SKD1; SKD1B; VPS4-2��
NCBI Protein Information:vacuolar protein sorting-associated protein 4B; cell migration-inducing 1; vacuolar protein sorting 4B; suppressor of K+ transport defect 1; cell migration-inducing gene 1 protein; suppressor of K(+) transport growth defect 1
UniProt Protein Name:Vacuolar protein sorting-associated protein 4B
UniProt Synonym Protein Names:Cell migration-inducing gene 1 protein; Suppressor of K(+) transport growth defect 1; Protein SKD1
Protein Family:Vacuolar protein sorting-associated protein
UniProt Gene Name:VPS4B��
UniProt Entry Name:VPS4B_HUMAN

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