Human Voltage Dependent Anion Channel Protein 1 (VDAC1) ELISA Kit
The Human Voltage-Dependent Anion Channel Protein 1 (VDAC1) ELISA Kit is a highly reliable and sensitive assay designed for the accurate quantification of VDAC1 levels in human samples such as serum, plasma, and cell culture supernatants. VDAC1 is a key protein involved in mitochondrial function and apoptosis regulation, making it a crucial target for research in areas such as cancer, neurodegenerative diseases, and metabolic disorders.This ELISA kit offers high specificity and precision, ensuring consistent and reproducible results for your experiments. By measuring VDAC1 levels, researchers can gain valuable insights into the role of this protein in various physiological and pathological processes, ultimately leading to a better understanding of disease mechanisms and potential therapeutic interventions.
Whether you are investigating the molecular mechanisms underlying mitochondrial dysfunction or exploring new therapeutic strategies for diseases involving VDAC1, this ELISA kit is an essential tool for your research endeavors. Trust in the accuracy and reliability of the Human VDAC1 ELISA Kit to advance your scientific discovery and contribute to the development of novel treatments for challenging medical conditions.
Product Name:
Human Voltage Dependent Anion Channel Protein 1 (VDAC1) ELISA Kit
Product Code:
HUDL03079
Size:
96 Assays
Target:
VDAC1
Synonyms:
PORIN-31-HL, Porin 31HM, Plasmalemmal porin, Outer mitochondrial membrane protein porin 1, Voltage-dependent anion-selective channel protein 1
Detection Method:
Sandwich
Range:
31.25-2,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
