The Human VF (Visfatin) ELISA Kit is specifically designed for the precise measurement of visfatin levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it perfect for various research purposes.Visfatin is an important protein that plays a key role in regulating glucose metabolism and insulin sensitivity. It is also involved in inflammation and immune response, making it a valuable biomarker for studying metabolic disorders, autoimmune diseases, and inflammatory conditions.
With its advanced technology and reliable performance, the Human VF (Visfatin) ELISA Kit is a valuable tool for researchers and clinicians working on understanding the role of visfatin in various diseases and developing potential therapeutic interventions.
Product Name:
Human VF (Visfatin) ELISA Kit
SKU:
HUES02741
Target:
Human VF (Visfatin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VF. You can calculate the concentration of Human VF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
84-96
93-105
Average (%)
93
91
98
1:4
Range (%)
93-106
84-95
85-101
Average (%)
99
89
92
1:8
Range (%)
88-101
84-97
84-96
Average (%)
93
90
90
1:16
Range (%)
89-103
85-100
84-97
Average (%)
95
91
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
95
EDTA plasma (n=5)
93-105
100
Cell culture media (n=5)
87-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.93
2.1
7.95
0.96
2.2
7.16
Standard deviation
0.06
0.09
0.33
0.05
0.09
0.26
C V (%)
6.45
4.29
4.15
5.21
4.09
3.63
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human VF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VF in samples. No significant cross-reactivity or interference between Human VF and analogues was observed.
