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Human Very Low Density Lipoprotein Receptor (VLDLR) ELISA Kit

SKU:
HUDL03100
Product Type:
ELISA Kit
Sensitivity:
0.57ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
VLDL-R
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

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Human Very Low Density Lipoprotein Receptor (VLDLR) ELISA Kit

The Human Very Low Density Lipoprotein Receptor (VLDLR) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of VLDLR levels in human serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it ideal for research applications related to lipid metabolism and cardiovascular health.The VLDLR protein is a key component in the regulation of lipid uptake and storage, as well as playing a role in the development of atherosclerosis and cardiovascular disease.

By measuring VLDLR levels, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify novel therapeutic targets.Overall, the Human VLDLR ELISA Kit provides a valuable tool for studying the role of VLDLR in lipid metabolism and cardiovascular health, offering researchers a reliable and accurate method for detecting and quantifying VLDLR levels in human samples.

Product Name:Human Very Low Density Lipoprotein Receptor (VLDLR) ELISA Kit
Product Code:HUDL03100
Size:96 Assays
Target:VLDLR
Synonyms:VLDL-R
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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