Human VEGFR-2/KDR (Vascular Endothelial Growth Factor Receptor 2) CLIA Kit
The Human VEGFR-2 (KDR) CLIA Kit is specifically developed for the precise measurement of Vascular Endothelial Growth Factor Receptor 2 (VEGFR-2) levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity for accurate and consistent results, making it an excellent choice for a variety of research applications.VEGFR-2 is a key receptor involved in the regulation of angiogenesis, which is crucial for the formation of new blood vessels. Dysregulation of VEGFR-2 signaling has been implicated in various diseases such as cancer, cardiovascular disorders, and inflammatory conditions.
Therefore, the measurement of VEGFR-2 levels can provide valuable insights into disease pathogenesis and aid in the development of targeted therapies.Overall, the Human VEGFR-2 (KDR) CLIA Kit is a reliable tool for researchers looking to study the role of VEGFR-2 in health and disease, offering accurate and reproducible results to advance scientific understanding and potential therapeutic interventions.
Product Name:
Human VEGFR-2/KDR (Vascular Endothelial Growth Factor Receptor 2) CLIA Kit
SKU:
HUES00913
Target:
Human VEGFR-2/KDR (Vascular Endothelial Growth Factor Receptor 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human VEGFR-2/KDR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VEGFR-2/KDR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VEGFR-2/KDR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human VEGFR-2/KDR. You can calculate the concentration of Human VEGFR-2/KDR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
91-104
89-104
Average (%)
92
96
97
1:4
Range (%)
96-112
98-109
91-107
Average (%)
102
103
98
1:8
Range (%)
102-117
87-101
91-102
Average (%)
108
94
97
1:16
Range (%)
102-119
101-118
91-101
Average (%)
109
108
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
97
EDTA plasma (n=5)
100-117
108
Cell culture media (n=5)
86-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.08
139.14
480.54
51.41
138.83
499.06
Standard deviation
5.13
14.86
52.62
5.07
15.05
32.89
C V (%)
10.24
10.68
10.95
9.86
10.84
6.59
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human VEGFR-2/KDR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VEGFR-2/KDR in samples. No significant cross-reactivity or interference between Human VEGFR-2/KDR and analogues was observed.
