Human VEGF165 (Vascular Endothelial Growth Factor165) CLIA Kit
The Human VEGF165 (Vascular Endothelial Growth Factor 165) CLIA Kit is specifically designed for the quantitative detection of VEGF165 levels in human serum and plasma samples. This kit offers high sensitivity and accuracy, providing researchers with reliable and reproducible results for a variety of research applications.VEGF165 is a key protein in angiogenesis, playing a vital role in the formation of new blood vessels. It is involved in various physiological and pathological processes, including tumor growth, wound healing, and cardiovascular diseases.
The measurement of VEGF165 levels can provide valuable insights into disease progression and response to treatment, making it a valuable tool for research and clinical applications.Overall, the Human VEGF165 CLIA Kit is a valuable resource for researchers studying angiogenesis and its role in various diseases. Its high sensitivity and specificity ensure accurate detection of VEGF165 levels, making it a reliable tool for advancing our understanding of angiogenesis and developing potential therapies.
Product Name:
Human VEGF165 (Vascular Endothelial Growth Factor165) CLIA Kit
SKU:
HUES01139
Target:
Human VEGF165 (Vascular Endothelial Growth Factor165)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human VEGF165. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VEGF165 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VEGF165, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human VEGF165. You can calculate the concentration of Human VEGF165 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
97-113
83-97
Average (%)
102
104
90
1:4
Range (%)
89-99
95-108
90-100
Average (%)
94
103
95
1:8
Range (%)
93-104
91-105
99-114
Average (%)
99
97
106
1:16
Range (%)
91-105
87-103
86-96
Average (%)
96
94
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-108
103
EDTA plasma (n=5)
87-101
93
Cell culture media (n=5)
92-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
55.46
141.92
423.07
52.49
150.92
436.78
Standard deviation
6.52
15.28
40.78
6.49
17.73
31.32
C V (%)
11.76
10.77
9.64
12.36
11.75
7.17
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human VEGF165 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VEGF165 in samples. No significant cross-reactivity or interference between Human VEGF165 and analogues was observed.
