Human Vasoactive Intestinal Peptide Receptor 1 (VIPR1) ELISA Kit
The Human Vasoactive Intestinal Peptide Receptor 1 (VIPR1) ELISA Kit is a reliable and accurate tool for the detection of VIPR1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.VIPR1 is a key receptor involved in mediating the effects of vasoactive intestinal peptide (VIP), a neuropeptide with diverse physiological functions including regulating smooth muscle relaxation, neurotransmission, and immune response. Dysregulation of VIPR1 signaling has been implicated in several diseases such as inflammatory disorders, neurological conditions, and gastrointestinal diseases, making it a valuable target for research and potential therapeutic interventions.
This ELISA kit provides researchers with a valuable tool to study VIPR1 biology, explore its role in disease mechanisms, and develop novel treatments targeting this receptor. With its user-friendly protocol and robust performance, the Human VIPR1 ELISA Kit is a valuable asset for laboratories conducting cutting-edge research in the field of neurobiology, immunology, and pharmacology.
Product Name:
Human Vasoactive Intestinal Peptide Receptor 1 (VIPR1) ELISA Kit
Product Code:
HUDL03097
Size:
96 Assays
Target:
VIPR1
Synonyms:
HVR1, PACAP-R-2, RDC1, VAPC1, VIPR, VIRG, VPAC1, Pituitary adenylate cyclase-activating polypeptide type II receptor
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
