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Human Vascular Endothelial Growth Factor Receptor 3 (VEGFR3) ELISA Kit

SKU:
HUDL03089
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P35916
Sensitivity:
0.125ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
FLT41,FLT4,PCL,Fms-Related Tyrosine Kinase 4,Tyrosine-protein kinase receptor FLT4
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€599
Frequently bought together:

Description

system_update_altDatasheet

Human Vascular Endothelial Growth Factor Receptor 3 (VEGFR3) ELISA Kit

The Human VEGFR3 (Vascular Endothelial Growth Factor Receptor 3) ELISA Kit is designed for the accurate detection of VEGFR3 levels in human serum, plasma, and cell culture supernatants. This kit features high sensitivity and specificity, ensuring reliable and reproducible results, making it ideal for a wide range of research applications.VEGFR3 is a critical receptor protein involved in angiogenesis, specifically lymphangiogenesis, playing a key role in the formation of lymphatic vessels.

Dysregulation of VEGFR3 has been implicated in various diseases, including cancer, lymphedema, and inflammatory disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions. With its easy-to-use format and robust performance, the Human VEGFR3 ELISA Kit is a valuable tool for researchers and clinicians seeking to explore the role of VEGFR3 in health and disease. Visit assaygenie.com to learn more and order your kit today.

Product Name:Human Vascular Endothelial Growth Factor Receptor 3 (VEGFR3) ELISA Kit
Product Code:HUDL03089
Size:96 Assays
Target:VEGFR3
Synonyms:FLT41, FLT4, PCL, Fms-Related Tyrosine Kinase 4, Tyrosine-protein kinase receptor FLT4
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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