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Human Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) ELISA Kit

SKU:
HUDL03087
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P17948
Sensitivity:
0.066ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
Flt1,Fms-Related Tyrosine Kinase 1,Vascular Permeability Factor Receptor,Fms-like tyrosine kinase 1,Tyrosine-protein kinase receptor FLT,
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Saliva, Cell Lysates, Follicular Fluid And Other Biological Fluids
€599
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Description

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Human Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) ELISA Kit

The Human Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) ELISA Kit is a cutting-edge tool for measuring VEGFR1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit boasts outstanding sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.VEGFR1 is a key receptor involved in angiogenesis, the process of new blood vessel formation.

It plays a crucial role in various diseases including cancer, cardiovascular disorders, and neurodegenerative conditions. By accurately measuring VEGFR1 levels, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially develop new therapeutic interventions. The Human VEGFR1 ELISA Kit is a vital resource for investigators studying angiogenesis and its impact on health and disease.

Product Name:Human Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) ELISA Kit
Product Code:HUDL03087
Size:96 Assays
Target:VEGFR1
Synonyms:Flt1, Fms-Related Tyrosine Kinase 1, Vascular Permeability Factor Receptor, Fms-like tyrosine kinase 1, Tyrosine-protein kinase receptor FLT
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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