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Human Vascular Endothelial Growth Factor 165 (VEGF165) ELISA Kit

SKU:
HUDL03082
Product Type:
ELISA Kit
Sensitivity:
5.7pg/mL
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
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Description

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Human Vascular Endothelial Growth Factor 165 (VEGF165) ELISA Kit

The Human VEGF165 (Vascular Endothelial Growth Factor 165) ELISA Kit is a powerful tool for detecting and quantifying VEGF165 levels in human biological samples. This kit is highly sensitive and specific, providing accurate and reliable results for research applications.VEGF165 is a key regulator of angiogenesis, stimulating the growth of new blood vessels and influencing various physiological processes. Abnormal levels of VEGF165 have been linked to diseases such as cancer, diabetes, and retinopathy, highlighting its importance as a biomarker for studying these conditions.

With its advanced technology and superior performance, the Human VEGF165 ELISA Kit is an essential tool for researchers investigating angiogenesis, vascular biology, and related disease pathways. Trust in this kit to deliver precise and consistent results for your research needs.

Product Name:Human Vascular Endothelial Growth Factor 165 (VEGF165) ELISA Kit
Product Code:HUDL03082
Size:96 Assays
Target:VEGF165
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10095
Heparin plasma (n=5)91-10196
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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