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Human Vanin 3 (VNN3) ELISA Kit

SKU:
HUDL03104
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9NY84
Sensitivity:
0.056ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
Vascular non-inflammatory molecule 3
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

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Human Vanin 3 (VNN3) ELISA Kit

The Human VNN3 (Vanin-3) ELISA Kit is a powerful tool for measuring vanin-3 levels in human biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results, making it suitable for a variety of research applications.Vanin-3 is an enzyme that plays a crucial role in oxidative stress response and inflammation regulation. Its expression has been implicated in various diseases, including metabolic disorders, inflammatory conditions, and cancer.

By measuring vanin-3 levels, researchers can gain valuable insights into the pathogenesis of these diseases and potentially identify new therapeutic targets.Overall, the Human VNN3 ELISA Kit is essential for scientists studying the role of vanin-3 in health and disease, offering a comprehensive and efficient solution for measuring its levels in human samples.

Product Name:Human Vanin 3 (VNN3) ELISA Kit
Product Code:HUDL03104
Size:96 Assays
Target:VNN3
Synonyms:Vascular non-inflammatory molecule 3
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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