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Human Valosin Containing Protein (VCP) ELISA Kit

SKU:
HUDL03078
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P55072
Sensitivity:
0.122ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
IBMPFD,p97,TERA,Transitional Endoplasmic Reticulum ATPase,15S Mg(2+)-ATPase p97 subunit
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

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Human Valosin Containing Protein (VCP) ELISA Kit

The Human Valosin-Containing Protein (VCP) ELISA Kit is a cutting-edge tool for accurately measuring VCP levels in human biological samples. This kit boasts exceptional sensitivity and specificity, delivering consistent and precise results for various research purposes. Valosin-Containing Protein, also known as p97, is a key player in cellular protein quality control processes, including protein degradation and clearance of misfolded proteins. Dysregulation of VCP has been linked to various diseases such as neurodegenerative disorders, muscle-wasting conditions, and certain cancers, making it a valuable target for biomarker research and drug development.

With the Human VCP ELISA Kit, researchers can gain valuable insights into the role of VCP in disease pathogenesis and potentially discover new therapeutic strategies. Trust in the accuracy and reliability of this ELISA kit to advance your research endeavors in the field of VCP biology.

Product Name:Human Valosin Containing Protein (VCP) ELISA Kit
Product Code:HUDL03078
Size:96 Assays
Target:VCP
Synonyms:IBMPFD, p97, TERA, Transitional Endoplasmic Reticulum ATPase, 15S Mg(2+)-ATPase p97 subunit
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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