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Human V-Set Domain Containing T-Cell Activation Inhibitor 1 (VTCN1) ELISA Kit

SKU:
HUDL03109
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q7Z7D3
Sensitivity:
0.054ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
B7-H4,B7H4,B7S1,B7X,B7h.5,VCTN1,T-cell costimulatory molecule B7x,Immune costimulatory protein B7-H4
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human V-Set Domain Containing T-Cell Activation Inhibitor 1 (VTCN1) ELISA Kit

The Human V-Set Domain Containing T-Cell Activation Inhibitor 1 (VTCN1) ELISA Kit is designed for the accurate measurement of VTCN1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.VTCN1, also known as B7-H4, is an important immune regulatory protein that plays a role in inhibiting T-cell activation and immune responses. It is involved in various inflammatory conditions, autoimmune diseases, and cancer, making it a valuable biomarker for studying and potentially targeting these diseases.

By utilizing the Human VTCN1 ELISA Kit, researchers can gain valuable insights into the role of VTCN1 in the immune system and its potential implications for therapeutic interventions. This kit provides a reliable and efficient tool for analyzing VTCN1 levels, contributing to the advancement of research in immunology and disease biology.

Product Name:Human V-Set Domain Containing T-Cell Activation Inhibitor 1 (VTCN1) ELISA Kit
Product Code:HUDL03109
Size:96 Assays
Target:VTCN1
Synonyms:B7-H4, B7H4, B7S1, B7X, B7h.5, VCTN1, T-cell costimulatory molecule B7x, Immune costimulatory protein B7-H4
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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