Human V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit
The Human V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit is a reliable and accurate tool for detecting levels of the MYC protein in human samples. This kit is specifically designed for use with serum, plasma, and cell culture supernatants, providing researchers with high sensitivity and specificity for consistent and dependable results.The MYC protein is a key regulator of cell growth and proliferation, playing a critical role in cancer development and progression. By measuring MYC levels, researchers can gain valuable insights into the mechanisms driving oncogenesis and potentially identify new targets for therapeutic intervention.
With its robust performance and versatility, the Human V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit is an essential tool for studying MYC biology and its implications in cancer research, cardiovascular diseases, neurodegenerative disorders, and beyond. Trust in this kit to accelerate your research and advance our understanding of MYC-related pathologies.
Product Name:
Human V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) ELISA Kit
Product Code:
HUDL01949
Size:
96 Assays
Target:
MYC
Synonyms:
C-Myc, BHLHE39, Myc Proto-Oncogene Protein, Class E basic helix-loop-helix protein 39, Transcription factor p64, Proto-oncogene c-Myc
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
