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Human V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (MAF) ELISA Kit

SKU:
HUDL01778
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O75444
Sensitivity:
0.074ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
c-MAF,Proto-oncogene c-Maf,Transcription factor Maf
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (MAF) ELISA Kit

The Human V-MAF Musculoaponeurotic Fibrosarcoma Oncogene Homolog (MAF) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of MAF levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it a valuable tool for a variety of research applications.MAF is a transcription factor that plays a critical role in regulating gene expression and is involved in various cellular processes, including differentiation, proliferation, and apoptosis.

Dysregulation of MAF has been linked to a range of diseases, including cancer, diabetes, and autoimmune disorders, making it an important target for investigation and potential therapeutic development.Whether studying the role of MAF in disease pathogenesis or exploring its potential as a diagnostic or prognostic biomarker, the Human V-MAF ELISA Kit offers researchers a powerful tool for advancing their understanding of this key protein.

Product Name:Human V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (MAF) ELISA Kit
Product Code:HUDL01778
Size:96 Assays
Target:MAF
Synonyms:c-MAF, Proto-oncogene c-Maf, Transcription factor Maf
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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