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Human Urotensin 2 Receptor (UTS2R) ELISA Kit

SKU:
HUDL03068
Product Type:
ELISA Kit
Sensitivity:
0.053ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
UT,UTS2-R,GPR14,UTR2,Urotensin-II Receptor,Hypocretin Receptor,G Protein-Coupled Receptor 14
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Urotensin 2 Receptor (UTS2R) ELISA Kit

The Human Urotensin-2 Receptor (UTS2R) ELISA Kit is specifically designed for the precise detection of UTS2R levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and reproducible results for a variety of research applications.UTS2R is a key receptor involved in the urotensin-2 signaling pathway, which has been implicated in various physiological and pathological processes, including cardiovascular diseases, renal disorders, and inflammation.

By accurately measuring UTS2R levels, researchers can gain valuable insights into the role of this receptor in disease development and progression, ultimately paving the way for potential therapeutic interventions.

Product Name:Human Urotensin 2 Receptor (UTS2R) ELISA Kit
Product Code:HUDL03068
Size:96 Assays
Target:UTS2R
Synonyms:UT, UTS2-R, GPR14, UTR2, Urotensin-II Receptor, Hypocretin Receptor, G Protein-Coupled Receptor 14
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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