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Human Urotensin-2 PharmaGenie ELISA Kit (SBRS0178)

SKU:
SBRS0178
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Competitive
€649
Frequently bought together:

Description

Human Urotensin-2 PharmaGenie ELISA Kit (SBRS0178)

Product SKU:SBRS0178
Size:96T
Application:This ELISA kit is designed to detect human, mouse, and rat Urotensin-2.
Uniprot:O95399 PRO_36347
Gene ID:10911
Gene Names:UNQ525/PRO1068 / UTS2
Synonyms:Urotensin-2 (Urotensin II) (U-II) (UII)
Target Species:Human, Mouse, Rat
Compatible Sample Types:Cell Culture Supernatants, Plasma, Serum
Design Principle:Competition-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Quantitative
Storage/Stability:Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
  1. Pre-Coated 96-well Strip Microplate
  2. Wash Buffer
  3. Standard Peptide
  4. Assay Diluent(s)
  5. Biotinylated Peptide
  6. HRP-Streptavidin
  7. TMB One-Step Substrate
  8. Stop Solution
  9. Assay Diagram
  10. Positive Control Sample
  11. Capture Antibody
  12. Technical Manual

Other materials and equipment required:

The Assay Genie Human Urotensin-2 PharmaGenie ELISA Kit (SBRS0178) will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Orbital shaker
  • Aluminum foil
  • Saran Wrap
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
  1. Prepare all reagents, samples and standards as instructed.
  2. Add 100 ul detection antibody to each well.
  3. Incubate 1.5 h at RT or O/N at 4°C.
  4. Add 100 ul standard or sample to each well.
  5. Incubate 2.5 h at RT.
  6. Add 100 ul prepared streptavidin solution.
  7. Incubate 45 min at RT.
  8. Add 100 ul TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul Stop Solution to each well.
  11. Read plate at 450 nm immediately.
Product ImageStandard Curve for Human Urotensin-2 PharmaGenie ELISA Kit (SBES0178)

Urotensin-II (U-II) is a 11 amino acids peptide ligand encoded by the UTS2 gene. It binds to a class of G protein-coupled receptor known as GPR14 or the urotensin receptor. It has been reported that Urotensin-II is involved in the cardiovascular, nervous, endocrine, pulmonary, renal and metabolic systems. Elevated plasma levels of U-II have been shown in numerous diseased conditions, such as hypertension, atherosclerosis and heart failure. U-II and its receptor represent promising targets for the development of new drugs.

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