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Human Uncoupling Protein 1, Mitochondrial (UCP1) ELISA Kit

SKU:
HUDL03043
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P25874
Sensitivity:
0.063ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
SLC25A7,UCP,Thermogenin,Solute carrier family 25 member 7
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Uncoupling Protein 1, Mitochondrial (UCP1) ELISA Kit

The Human Uncoupling Protein 1 Mitochondrial (UCP1) ELISA Kit is a powerful tool for accurately measuring UCP1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research settings.UCP1 is a key protein involved in thermogenesis and energy expenditure, particularly in brown adipose tissue. Its expression is crucial in the regulation of metabolism and heat production, making it a valuable marker for studying metabolic disorders such as obesity and diabetes.

With reliable data provided by the Human UCP1 ELISA Kit, researchers can gain important insights into the role of UCP1 in metabolic processes and potentially identify new therapeutic targets for managing metabolic diseases. Elevate your research with this cutting-edge ELISA kit from AssayGenie.

Product Name:Human Uncoupling Protein 1, Mitochondrial (UCP1) ELISA Kit
Product Code:HUDL03043
Size:96 Assays
Target:UCP1
Synonyms:SLC25A7, UCP, Thermogenin, Solute carrier family 25 member 7
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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