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Human UL16 Binding Brotein 2 (ULBP2) ELISA Kit

SKU:
HUDL03051
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9BZM5
Sensitivity:
0.055ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
N2DL2,RAET1H,NKG2D Ligand 2,Retinoic acid early transcript 1H,NKG2D ligand 2
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
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Description

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Human UL16 Binding Brotein 2 (ULBP2) ELISA Kit

The Human UL16-Binding Protein 2 (ULBP2) ELISA Kit is specially designed for the precise detection of ULBP2 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results, making it an excellent choice for various research applications.ULBP2 is a key protein involved in immune response regulation and is known to play a crucial role in immune surveillance against tumor cells.

Its expression levels have been linked to various cancers and autoimmune diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions. With its reliable performance and ease of use, the Human UL16-Binding Protein 2 (ULBP2) ELISA Kit is a valuable tool for researchers looking to explore the role of ULBP2 in health and disease.

Product Name:Human UL16 Binding Brotein 2 (ULBP2) ELISA Kit
Product Code:HUDL03051
Size:96 Assays
Target:ULBP2
Synonyms:N2DL2, RAET1H, NKG2D Ligand 2, Retinoic acid early transcript 1H, NKG2D ligand 2
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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