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Human UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1) ELISA Kit

SKU:
HUDL03048
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P22309
Sensitivity:
0.51ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
UGT1,GNT1,UGT1A,UDPGT,HUG-BR1,UGT1-1,Bilirubin-specific UDPGT isozyme 1,UDP-glucuronosyltransferase 1-1
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

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Human UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1) ELISA Kit

The Human UDP Glucuronosyltransferase 1 Family Polypeptide A1 (UGT1A1) ELISA Kit is specifically designed for the precise measurement of UGT1A1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.UGT1A1 is a key enzyme responsible for the detoxification and elimination of a wide range of endogenous and exogenous compounds through glucuronidation.

Dysregulation of UGT1A1 activity has been implicated in various diseases, including Gilbert syndrome and drug-induced liver injury, highlighting its significance as a potential biomarker for studying these conditions and developing targeted treatments.Overall, the Human UGT1A1 ELISA Kit serves as a valuable tool for researchers interested in exploring the role of UGT1A1 in health and disease, providing a reliable and efficient method for measuring UGT1A1 levels in human biological samples.

Product Name:Human UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1) ELISA Kit
Product Code:HUDL03048
Size:96 Assays
Target:UGT1A1
Synonyms:UGT1, GNT1, UGT1A, UDPGT, HUG-BR1, UGT1-1, Bilirubin-specific UDPGT isozyme 1, UDP-glucuronosyltransferase 1-1
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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