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Human UCK1 Recombinant Protein (RPPB2675)

SKU:
RPPB2675
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9HA47
Research Area:
Enzymes
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Description

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Product Name:Human UCK1 Recombinant Protein
Product Code:RPPB2675
Size:25µg
Species:Human
Target:UCK1
Synonyms:Uridine-cytidine kinase 1, UCK 1, Cytidine monophosphokinase 1, UCK1 Uridine monophosphokinase 1, URK1, FLJ12255, RP11-334J6.5, Uridine-cytidine kinase 1 isoform a.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:UCK1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.4M Urea.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 80.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MFYSQEIRDM FHLRLFVDTD SDVRLSRRVL RDVRRGRDLE QILTQYTTFV KPAFEEFCLP TKKYADVIIP RGVDNMVAIN LIVQHIQDIL NGDICKWHRG GSNGRSYKRT FSEPGDHPGM LTSGKRSHLE SS

UCK1 is a uridine-cytidine kinase which catalyzes the phosphorylation of uridine and cytidine to uridine monophosphate and cytidine monophosphate. UCK1 does not phosphorylate deoxyribonucleosides or purine ribonucleosides. UCK1 is also phosphorylates uridine and cytidine analogs and uses ATP and GTP as a phosphate donor.

UCK1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids (143-273a.a) and having a molecular mass of 17.5kDa.UCK1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:UCK1: Phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. Does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Can use ATP or GTP as a phosphate donor. Can also phosphorylate cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4- thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)- benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5- methylcytidine, and N(4)-anisoylcytidine. Belongs to the uridine kinase family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Kinase, other; Nucleotide Metabolism - pyrimidine; Xenobiotic Metabolism - drug metabolism - other enzymes; EC 2.7.1.48Chromosomal Location of Human Ortholog: 9q34.13Cellular Component: cytosolMolecular Function: nucleoside kinase activity; uridine kinase activityBiological Process: pyrimidine base metabolic process; pyrimidine nucleoside salvage
UniProt Protein Details:
NCBI Summary:This gene encodes a uridine-cytidine kinase that catalyzes the phosphorylation of uridine and cytidine to uridine monophosphate (UMP) and cytidine monophosphate (CMP) but not the phosphorylation of deoxyribonucleosides or purine ribonucleosides. This enzyme can also phosphorylate uridine and cytidine analogs and uses both ATP and GTP as a phosphate donor. Alternative splicing results in multiple splice variants encoding distinct isoforms. [provided by RefSeq, May 2012]
UniProt Code:Q9HA47
NCBI GenInfo Identifier:20455360
NCBI Gene ID:83549
NCBI Accession:Q9HA47.1
UniProt Secondary Accession:Q9HA47,Q5JT09, Q5JT10, Q5JT12, Q5JT13, Q6IA74, Q96BJ0
UniProt Related Accession:Q9HA47
Molecular Weight:32,275 Da
NCBI Full Name:Uridine-cytidine kinase 1
NCBI Synonym Full Names:uridine-cytidine kinase 1
NCBI Official Symbol:UCK1��
NCBI Official Synonym Symbols:URK1��
NCBI Protein Information:uridine-cytidine kinase 1
UniProt Protein Name:Uridine-cytidine kinase 1
UniProt Synonym Protein Names:Cytidine monophosphokinase 1; Uridine monophosphokinase 1
Protein Family:Uridine-cytidine kinase
UniProt Gene Name:UCK1��
UniProt Entry Name:UCK1_HUMAN

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