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Human Ubiquitin-like protein 5 PharmaGenie ELISA Kit (SBRS0176)

SKU:
SBRS0176
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Competitive
$1,235

Description

Human Ubiquitin-like protein 5 PharmaGenie ELISA Kit (SBRS0176)

Product SKU:SBRS0176
Size:96T
Application:This ELISA kit is designed to detect human, mouse, and rat Ubiquitin-like protein 5.
Uniprot:Q9BZL1
Gene ID:59286
Gene Names:UBL5
Synonyms:Ubiquitin-like protein 5
Target Species:Human, Mouse, Rat
Compatible Sample Types:Cell Culture Supernatants, Serum
Design Principle:Competition-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Quantitative
Range:0.1-1,000 ng/ml
Sensitivity:0.1 ng/ml
Recommended Dilution:Human: 2x, Mouse: 2x, Rat: 2x
Storage/Stability:Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
  1. Pre-Coated 96-well Strip Microplate
  2. Wash Buffer
  3. Standard Peptide
  4. Assay Diluent(s)
  5. Biotinylated Peptide
  6. HRP-Streptavidin
  7. TMB One-Step Substrate
  8. Stop Solution
  9. Assay Diagram
  10. Positive Control Sample
  11. Capture Antibody
  12. Technical Manual

Other materials and equipment required:

The Assay Genie Human Ubiquitin-like protein 5 PharmaGenie ELISA Kit (SBRS0176) will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Orbital shaker
  • Aluminum foil
  • Saran Wrap
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
  1. Prepare all reagents, samples and standards as instructed.
  2. Add 100 ul detection antibody to each well.
  3. Incubate 1.5 h at RT or O/N at 4°C.
  4. Add 100 ul standard or sample to each well.
  5. Incubate 2.5 h at RT.
  6. Add 100 ul prepared streptavidin solution.
  7. Incubate 45 min at RT.
  8. Add 100 ul TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul Stop Solution to each well.
  11. Read plate at 450 nm immediately.
Product ImageStandard Curve for Human Ubiquitin-like protein 5 PharmaGenie ELISA Kit (SBES0176)

Ubiquitin-like proteins (UBLs), a group of proteins similar to ubiquitin, are thought to be reversible modulators of protein function rather than protein degraders like ubiquitin. They are enzymatically conjugated via an isopeptide bond between the C-terminal glycine carboxylate group and a lysine residue on the target protein. However, UBL5, which is 64% identical to yeast Hub1, lacks glycine residues at its C terminus. UBL5 was found to complement Hub1 deletion mutants and rescued the mating projection defect. It has been studied as a link to predisposition to obesity based on its expression in Psammomys obesus, the fat sand rat, which is an animal model for obesity studies.

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