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Human Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ELISA Kit

SKU:
HUDL03039
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P09936
Sensitivity:
0.056ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
PARK5,PGP9.5,Uch-L1,Neuron cytoplasmic protein 9.5,Ubiquitin thioesterase L1,Ubiquitin carboxyl-terminal hydrolase isozyme L1
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

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Human Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ELISA Kit

The Human Ubiquitin Carboxyl-Terminal Hydrolase L1 (UCHL1) ELISA Kit is specifically designed for the accurate detection of UCHL1 levels in human serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, providing reliable and reproducible results for a variety of research applications.UCHL1 is an important enzyme that plays a key role in protein degradation and post-translational modification processes. Dysregulation of UCHL1 has been linked to various neurodegenerative disorders, making it a valuable biomarker for studying these conditions and potentially developing new therapies.

With the Human UCHL1 ELISA Kit, researchers can confidently measure UCHL1 levels in biological samples, leading to a better understanding of its function and potential implications in disease pathology. This kit is an essential tool for researchers looking to investigate the role of UCHL1 in health and disease.

Product Name:Human Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ELISA Kit
Product Code:HUDL03039
Size:96 Assays
Target:UCHL1
Synonyms:PARK5, PGP9.5, Uch-L1, Neuron cytoplasmic protein 9.5, Ubiquitin thioesterase L1, Ubiquitin carboxyl-terminal hydrolase isozyme L1
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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