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Human Ubiquitin Associated Protein 2 (UBAP2) ELISA Kit

SKU:
HUDL03029
Product Type:
ELISA Kit
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
$779
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Description

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Human Ubiquitin Associated Protein 2 (UBAP2) ELISA Kit

The Human Ubiquitin Associated Protein 2 (UBAP2) ELISA Kit is a powerful tool for quantifying UBAP2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is specifically designed for high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.UBAP2 is a key player in ubiquitin-mediated processes, playing a crucial role in protein degradation and cellular homeostasis.

Dysregulation of UBAP2 has been linked to various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions. Therefore, this ELISA kit is essential for studying UBAP2's role in these pathologies and developing targeted therapies.Overall, the Human UBAP2 ELISA Kit offers researchers a reliable and effective tool for investigating UBAP2 biology and its implications in disease mechanisms.

Product Name:Human Ubiquitin Associated Protein 2 (UBAP2) ELISA Kit
Product Code:HUDL03029
Size:96 Assays
Target:UBAP2
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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