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Human Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2) ELISA Kit

SKU:
HUDL03058
Product Type:
ELISA Kit
Sensitivity:
0.131ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
QCR2,UQCR2,Cytochrome b-c1 complex subunit 2, mitochondrial,Complex III subunit 2,Core protein II
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

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Human Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2) ELISA Kit

The Human Ubiquinol-Cytochrome C Reductase Core Protein II (UQCRC2) ELISA Kit is a reliable and accurate tool for detecting levels of UQCRC2 in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring consistent and precise results for a variety of research applications.UQCRC2 is a key protein in the Ubiquinol-Cytochrome C Reductase complex involved in the electron transport chain, essential for cellular energy production. Dysregulation of UQCRC2 has been linked to various diseases including metabolic disorders, neurodegenerative diseases, and cardiovascular conditions.

Therefore, this ELISA kit is a valuable resource for studying these conditions and exploring potential therapeutic interventions.Overall, the Human Ubiquinol-Cytochrome C Reductase Core Protein II (UQCRC2) ELISA Kit is a crucial tool for researchers looking to investigate the role of UQCRC2 in health and disease, offering precise and reliable results for advanced scientific analysis.

Product Name:Human Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2) ELISA Kit
Product Code:HUDL03058
Size:96 Assays
Target:UQCRC2
Synonyms:QCR2, UQCR2, Cytochrome b-c1 complex subunit 2, mitochondrial, Complex III subunit 2, Core protein II
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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