Human UBE2M Recombinant Protein (RPPB2386)
- SKU:
- RPPB2386
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P61081
- Research Area:
- Enzymes
Description
| Product Name: | Human UBE2M Recombinant Protein |
| Product Code: | RPPB2386 |
| Size: | 20µg |
| Species: | Human |
| Target: | UBE2M |
| Synonyms: | NEDD8-conjugating enzyme Ubc12, Ubiquitin-conjugating enzyme E2 M, NEDD8 protein ligase, NEDD8 carrier protein, UBC12, hUbc12, UBC-RS2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The UBE2M solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 1mM DTT and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMIKLFS LKQQKKEEES AGGTKGSSKK ASAAQLRIQK DINELNLPKT CDISFSDPDD LLNFKLVICP DEGFYKSGKF VFSFKVGQGY PHDPPKVKCE TMVYHPNIDL EGNVCLNILR EDWKPVLTIN SIIYGLQYLF LEPNPEDPLN KEAAEVLQNN RRLFEQNVQR SMRGGYIGST YFERCLK |
UbcH12 is functional in in vitro NEDDylation reactions. It has been shown to form a thioester linkage with NEDD8 in the presence of the NEDD8 activating enzyme complex Uba3/APP-BP1. APP-BP1 binds to the amyloid precursor protein (APP) carboxy terminal domain and is important in conjunction with Uba3 and UbcH12 in driving cells through the S to M checkpoint. NEDDylation of Cul-1 is essential for conjugation and processing of NF-kB p105 by SCF (b-TRCP) following phosphorylation of the complex. A dominant negative form of UbcH12, previously demonstrated to sequester NEDD8 and inhibit its conjugation, inhibits both conjugation and processing of p105, which is alleviated by wild-type UbcH12.
UBE2M Human Recombinant produced E. coli is a single polypeptide chain containing 207 amino acids (1-183) and having a molecular mass of 23.5kDa.UBE2M is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation. |
| NCBI Summary: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P61081 |
| NCBI GenInfo Identifier: | 4507791 |
| NCBI Gene ID: | 9040 |
| NCBI Accession: | NP_003960.1 |
| UniProt Secondary Accession: | P61081,O76069, Q8VC50, |
| UniProt Related Accession: | P61081 |
| Molecular Weight: | Observed: 21 kDaPredicted: 21 kDa |
| NCBI Full Name: | NEDD8-conjugating enzyme Ubc12 |
| NCBI Synonym Full Names: | ubiquitin conjugating enzyme E2 M |
| NCBI Official Symbol: | UBE2M�� |
| NCBI Official Synonym Symbols: | UBC12; hUbc12; UBC-RS2�� |
| NCBI Protein Information: | NEDD8-conjugating enzyme Ubc12 |
| UniProt Protein Name: | NEDD8-conjugating enzyme Ubc12 |
| UniProt Synonym Protein Names: | NEDD8 carrier protein; Ubiquitin-conjugating enzyme E2 M |
| UniProt Gene Name: | UBE2M�� |
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