Human UBE2I Recombinant Protein (RPPB2379)
- SKU:
- RPPB2379
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P63279
- Research Area:
- Enzymes
Description
| Product Name: | Human UBE2I Recombinant Protein |
| Product Code: | RPPB2379 |
| Size: | 50µg |
| Species: | Human |
| Target: | UBE2I |
| Synonyms: | SUMO-conjugating enzyme UBC9, EC 6.3.2.-, SUMO-protein ligase, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, Ubiquitin carrier protein I, Ubiquitin carrier protein 9, p18, UBC9, C358B7.1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The protein (1 mg/ml) contains 50mM HEPES (pH7.5) 150mM NaCl, 1mM DTT, and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | MSGIALSRLA QERKAWRKDH PFGFVAVPTK NPDGTMNLMN WECAIPGKKG TPWEGGLFKL RMLFKDDYPS SPPKCKFEPP LFHPNVYPSG TVCLSILEED KDWRPAITIK QILLGIQELL NEPNIQDPAQ AEAYTIYCQN RVEYEKRVRA QAKKFAPS |
Human Ubc9 is homologous to ubiquitin-conjugating enzymes (E2s). However, instead of conjugating ubiquitin, it conjugates a ubiquitin homologue, small ubiquitin-like modifier 1(SUMO-1). And hUbc9 retains striking structural and functional conservation with yeast Ubc9. The ubiquitin-dependent protein degradation system has been recognized as a complete enzymatic pathway that is responsible for the selective degradation of abnormal and short-lived proteins. The conjugation of ubiquitin requires the activities of ubiquitin-activating (E1) and �conjugating (E2) enzymes.
Ubiquitin-Conjugating Enzyme E2I Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 154 amino acids & having a molecular mass of 17.9 kDa.
| UniProt Protein Function: | Function: E2 ubiquitin-like--protein ligase mediating SUMO/Smt3 attachment to septins and PCNA. Seems to be involved in degradation of S- (CLB5) and M-phase cyclins (CLB2). Ref.4 Ref.5 |
| UniProt Protein Details: | Catalytic activity: ATP + SUMO + protein lysine = AMP + diphosphate + protein N-SUMOyllysine. Pathway: Protein modification; protein sumoylation. Subunit structure: Interacts with SIZ1. Ref.5 Ref.6 Subcellular location: Nucleus Ref.7. Miscellaneous: Present with 2600 molecules/cell in log phase SD medium. Ref.8 Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family. |
| UniProt Code: | P63279 |
| NCBI GenInfo Identifier: | |
| NCBI Gene ID: | |
| NCBI Accession: | |
| Molecular Weight: | 17,911 Da |
| NCBI Full Name: | UBC9 |
| UniProt Protein Name: | SUMO-conjugating enzyme UBC9 |
| UniProt Synonym Protein Names: | Ubiquitin carrier protein 9; Ubiquitin-conjugating enzyme E2-18 kDa; Ubiquitin-protein ligase |
| UniProt Gene Name: | UBC9�� |
| UniProt Entry Name: | UBC9_YEAST |
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